Staining:Article Title: Psoralen Enhances Direct Cardiac Reprogramming via PPARα Activation and Promotion of Mitochondrial Fission
Article Snippet: .. The materials used included: DMEM/F12 (1:1) basal medium (1X) (Gibco, 6123073), DMEM basic medium (1X) (Gibco, 6125275), fetal bovine serum (FBS, Procell, 164210-50), MEM non-essential amino acid (Servicebio, G4219), Sodium L-Ascorbyl-2-Phosphate (OriLeaf, S67343), Insulin-Transferrin-Selenium (ITS) G supplement (Servicebio, G4028), Cardiac Troponin T Polyclonal antibody (Protientech, 15513-1-AP), Alpha Actin Polyclonal antibody (Protientech, 23660-1-AP), Beta-actin polyclonal antibody (Protientech, 20536-1-AP), PPARA Monoclonal antibody (Protientech, 66826-1-Ig), RXRG Polyclonal antibody (Protientech, 11129-1-AP), UCP1 Polyclonal antibody (Protientech, 23673-1-AP), DRP1 (C-terminal) Polyclonal antibody (Protientech, 12957-1-AP), FIS1 Polyclonal antibody (Protientech, 10956-1-AP), DRP1 (phospho S616) Monoclonal antibody [EPR27387-57] (abcam, ab314755), Alexa Fluor 594-labeled goat anti-rabbit IgG (Servicebio, GB28301), Alexa Fluor 488-labeled goat anti-rabbit IgG (Servicebio, GB25303), Alexa Fluor 488-labeled goat anti-mouse IgG (Servicebio, GB25301), DAPI staining reagent (Servicebio, G1012), Fluo-4 AM (ThermoFisher, F14201), Mito-Tracker Red CMXRos (Beyotime, C1035), Hoechst 33342 (Solarbio, B8040), First Strand cDNA Synthesis Kit (RNase H minus, Beyotime, D7168M), RepSox (GLPBIO, GC16793), Forskolin (GLPBIO, GC11920), Psr (Beijing Bethealth People Biomedical Technology Co.,Ltd, 23010802), One-Step Western Kit HRP (CWBio, CW2029M), high-strength RIPA buffer (Solarbio, R0010-20ml), BCA Protein Assay Kit (Solarbio, PC0020), SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577), and qPCR SYBR Green Master Mix (High Rox Plus, Yeasen, 11203ES08). .. Primers were synthesized by Sangon Biotech.
cDNA Synthesis:Article Title: Psoralen Enhances Direct Cardiac Reprogramming via PPARα Activation and Promotion of Mitochondrial Fission
Article Snippet: .. The materials used included: DMEM/F12 (1:1) basal medium (1X) (Gibco, 6123073), DMEM basic medium (1X) (Gibco, 6125275), fetal bovine serum (FBS, Procell, 164210-50), MEM non-essential amino acid (Servicebio, G4219), Sodium L-Ascorbyl-2-Phosphate (OriLeaf, S67343), Insulin-Transferrin-Selenium (ITS) G supplement (Servicebio, G4028), Cardiac Troponin T Polyclonal antibody (Protientech, 15513-1-AP), Alpha Actin Polyclonal antibody (Protientech, 23660-1-AP), Beta-actin polyclonal antibody (Protientech, 20536-1-AP), PPARA Monoclonal antibody (Protientech, 66826-1-Ig), RXRG Polyclonal antibody (Protientech, 11129-1-AP), UCP1 Polyclonal antibody (Protientech, 23673-1-AP), DRP1 (C-terminal) Polyclonal antibody (Protientech, 12957-1-AP), FIS1 Polyclonal antibody (Protientech, 10956-1-AP), DRP1 (phospho S616) Monoclonal antibody [EPR27387-57] (abcam, ab314755), Alexa Fluor 594-labeled goat anti-rabbit IgG (Servicebio, GB28301), Alexa Fluor 488-labeled goat anti-rabbit IgG (Servicebio, GB25303), Alexa Fluor 488-labeled goat anti-mouse IgG (Servicebio, GB25301), DAPI staining reagent (Servicebio, G1012), Fluo-4 AM (ThermoFisher, F14201), Mito-Tracker Red CMXRos (Beyotime, C1035), Hoechst 33342 (Solarbio, B8040), First Strand cDNA Synthesis Kit (RNase H minus, Beyotime, D7168M), RepSox (GLPBIO, GC16793), Forskolin (GLPBIO, GC11920), Psr (Beijing Bethealth People Biomedical Technology Co.,Ltd, 23010802), One-Step Western Kit HRP (CWBio, CW2029M), high-strength RIPA buffer (Solarbio, R0010-20ml), BCA Protein Assay Kit (Solarbio, PC0020), SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577), and qPCR SYBR Green Master Mix (High Rox Plus, Yeasen, 11203ES08). .. Primers were synthesized by Sangon Biotech.
Western Blot:Article Title: Psoralen Enhances Direct Cardiac Reprogramming via PPARα Activation and Promotion of Mitochondrial Fission
Article Snippet: .. The materials used included: DMEM/F12 (1:1) basal medium (1X) (Gibco, 6123073), DMEM basic medium (1X) (Gibco, 6125275), fetal bovine serum (FBS, Procell, 164210-50), MEM non-essential amino acid (Servicebio, G4219), Sodium L-Ascorbyl-2-Phosphate (OriLeaf, S67343), Insulin-Transferrin-Selenium (ITS) G supplement (Servicebio, G4028), Cardiac Troponin T Polyclonal antibody (Protientech, 15513-1-AP), Alpha Actin Polyclonal antibody (Protientech, 23660-1-AP), Beta-actin polyclonal antibody (Protientech, 20536-1-AP), PPARA Monoclonal antibody (Protientech, 66826-1-Ig), RXRG Polyclonal antibody (Protientech, 11129-1-AP), UCP1 Polyclonal antibody (Protientech, 23673-1-AP), DRP1 (C-terminal) Polyclonal antibody (Protientech, 12957-1-AP), FIS1 Polyclonal antibody (Protientech, 10956-1-AP), DRP1 (phospho S616) Monoclonal antibody [EPR27387-57] (abcam, ab314755), Alexa Fluor 594-labeled goat anti-rabbit IgG (Servicebio, GB28301), Alexa Fluor 488-labeled goat anti-rabbit IgG (Servicebio, GB25303), Alexa Fluor 488-labeled goat anti-mouse IgG (Servicebio, GB25301), DAPI staining reagent (Servicebio, G1012), Fluo-4 AM (ThermoFisher, F14201), Mito-Tracker Red CMXRos (Beyotime, C1035), Hoechst 33342 (Solarbio, B8040), First Strand cDNA Synthesis Kit (RNase H minus, Beyotime, D7168M), RepSox (GLPBIO, GC16793), Forskolin (GLPBIO, GC11920), Psr (Beijing Bethealth People Biomedical Technology Co.,Ltd, 23010802), One-Step Western Kit HRP (CWBio, CW2029M), high-strength RIPA buffer (Solarbio, R0010-20ml), BCA Protein Assay Kit (Solarbio, PC0020), SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577), and qPCR SYBR Green Master Mix (High Rox Plus, Yeasen, 11203ES08). .. Primers were synthesized by Sangon Biotech.
Bicinchoninic Acid Protein Assay:Article Title: Psoralen Enhances Direct Cardiac Reprogramming via PPARα Activation and Promotion of Mitochondrial Fission
Article Snippet: .. The materials used included: DMEM/F12 (1:1) basal medium (1X) (Gibco, 6123073), DMEM basic medium (1X) (Gibco, 6125275), fetal bovine serum (FBS, Procell, 164210-50), MEM non-essential amino acid (Servicebio, G4219), Sodium L-Ascorbyl-2-Phosphate (OriLeaf, S67343), Insulin-Transferrin-Selenium (ITS) G supplement (Servicebio, G4028), Cardiac Troponin T Polyclonal antibody (Protientech, 15513-1-AP), Alpha Actin Polyclonal antibody (Protientech, 23660-1-AP), Beta-actin polyclonal antibody (Protientech, 20536-1-AP), PPARA Monoclonal antibody (Protientech, 66826-1-Ig), RXRG Polyclonal antibody (Protientech, 11129-1-AP), UCP1 Polyclonal antibody (Protientech, 23673-1-AP), DRP1 (C-terminal) Polyclonal antibody (Protientech, 12957-1-AP), FIS1 Polyclonal antibody (Protientech, 10956-1-AP), DRP1 (phospho S616) Monoclonal antibody [EPR27387-57] (abcam, ab314755), Alexa Fluor 594-labeled goat anti-rabbit IgG (Servicebio, GB28301), Alexa Fluor 488-labeled goat anti-rabbit IgG (Servicebio, GB25303), Alexa Fluor 488-labeled goat anti-mouse IgG (Servicebio, GB25301), DAPI staining reagent (Servicebio, G1012), Fluo-4 AM (ThermoFisher, F14201), Mito-Tracker Red CMXRos (Beyotime, C1035), Hoechst 33342 (Solarbio, B8040), First Strand cDNA Synthesis Kit (RNase H minus, Beyotime, D7168M), RepSox (GLPBIO, GC16793), Forskolin (GLPBIO, GC11920), Psr (Beijing Bethealth People Biomedical Technology Co.,Ltd, 23010802), One-Step Western Kit HRP (CWBio, CW2029M), high-strength RIPA buffer (Solarbio, R0010-20ml), BCA Protein Assay Kit (Solarbio, PC0020), SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577), and qPCR SYBR Green Master Mix (High Rox Plus, Yeasen, 11203ES08). .. Primers were synthesized by Sangon Biotech.
Real-time Polymerase Chain Reaction:Article Title: Psoralen Enhances Direct Cardiac Reprogramming via PPARα Activation and Promotion of Mitochondrial Fission
Article Snippet: .. The materials used included: DMEM/F12 (1:1) basal medium (1X) (Gibco, 6123073), DMEM basic medium (1X) (Gibco, 6125275), fetal bovine serum (FBS, Procell, 164210-50), MEM non-essential amino acid (Servicebio, G4219), Sodium L-Ascorbyl-2-Phosphate (OriLeaf, S67343), Insulin-Transferrin-Selenium (ITS) G supplement (Servicebio, G4028), Cardiac Troponin T Polyclonal antibody (Protientech, 15513-1-AP), Alpha Actin Polyclonal antibody (Protientech, 23660-1-AP), Beta-actin polyclonal antibody (Protientech, 20536-1-AP), PPARA Monoclonal antibody (Protientech, 66826-1-Ig), RXRG Polyclonal antibody (Protientech, 11129-1-AP), UCP1 Polyclonal antibody (Protientech, 23673-1-AP), DRP1 (C-terminal) Polyclonal antibody (Protientech, 12957-1-AP), FIS1 Polyclonal antibody (Protientech, 10956-1-AP), DRP1 (phospho S616) Monoclonal antibody [EPR27387-57] (abcam, ab314755), Alexa Fluor 594-labeled goat anti-rabbit IgG (Servicebio, GB28301), Alexa Fluor 488-labeled goat anti-rabbit IgG (Servicebio, GB25303), Alexa Fluor 488-labeled goat anti-mouse IgG (Servicebio, GB25301), DAPI staining reagent (Servicebio, G1012), Fluo-4 AM (ThermoFisher, F14201), Mito-Tracker Red CMXRos (Beyotime, C1035), Hoechst 33342 (Solarbio, B8040), First Strand cDNA Synthesis Kit (RNase H minus, Beyotime, D7168M), RepSox (GLPBIO, GC16793), Forskolin (GLPBIO, GC11920), Psr (Beijing Bethealth People Biomedical Technology Co.,Ltd, 23010802), One-Step Western Kit HRP (CWBio, CW2029M), high-strength RIPA buffer (Solarbio, R0010-20ml), BCA Protein Assay Kit (Solarbio, PC0020), SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577), and qPCR SYBR Green Master Mix (High Rox Plus, Yeasen, 11203ES08). .. Primers were synthesized by Sangon Biotech.
SYBR Green Assay:Article Title: Psoralen Enhances Direct Cardiac Reprogramming via PPARα Activation and Promotion of Mitochondrial Fission
Article Snippet: .. The materials used included: DMEM/F12 (1:1) basal medium (1X) (Gibco, 6123073), DMEM basic medium (1X) (Gibco, 6125275), fetal bovine serum (FBS, Procell, 164210-50), MEM non-essential amino acid (Servicebio, G4219), Sodium L-Ascorbyl-2-Phosphate (OriLeaf, S67343), Insulin-Transferrin-Selenium (ITS) G supplement (Servicebio, G4028), Cardiac Troponin T Polyclonal antibody (Protientech, 15513-1-AP), Alpha Actin Polyclonal antibody (Protientech, 23660-1-AP), Beta-actin polyclonal antibody (Protientech, 20536-1-AP), PPARA Monoclonal antibody (Protientech, 66826-1-Ig), RXRG Polyclonal antibody (Protientech, 11129-1-AP), UCP1 Polyclonal antibody (Protientech, 23673-1-AP), DRP1 (C-terminal) Polyclonal antibody (Protientech, 12957-1-AP), FIS1 Polyclonal antibody (Protientech, 10956-1-AP), DRP1 (phospho S616) Monoclonal antibody [EPR27387-57] (abcam, ab314755), Alexa Fluor 594-labeled goat anti-rabbit IgG (Servicebio, GB28301), Alexa Fluor 488-labeled goat anti-rabbit IgG (Servicebio, GB25303), Alexa Fluor 488-labeled goat anti-mouse IgG (Servicebio, GB25301), DAPI staining reagent (Servicebio, G1012), Fluo-4 AM (ThermoFisher, F14201), Mito-Tracker Red CMXRos (Beyotime, C1035), Hoechst 33342 (Solarbio, B8040), First Strand cDNA Synthesis Kit (RNase H minus, Beyotime, D7168M), RepSox (GLPBIO, GC16793), Forskolin (GLPBIO, GC11920), Psr (Beijing Bethealth People Biomedical Technology Co.,Ltd, 23010802), One-Step Western Kit HRP (CWBio, CW2029M), high-strength RIPA buffer (Solarbio, R0010-20ml), BCA Protein Assay Kit (Solarbio, PC0020), SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34577), and qPCR SYBR Green Master Mix (High Rox Plus, Yeasen, 11203ES08). .. Primers were synthesized by Sangon Biotech.
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